《植物生理学报》 2010, 46(6): 555-558

利用农杆菌介导的瞬时表达系统研究CYC 类TCP 蛋白的亚细胞定位

李超¹, 许治永², 杨军², 罗达^1,2,*

1 上海交通大学生命科学技术学院, 上海 200240; 2 中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家 重点实验室, 上海 200032

通信作者：罗达；E-mail: dluo@sibs.ac.cn；Tel: 021-54924108

摘　要：

CYCLOIDEA (CYC)类 TCP 基因在豆科与玄参科植物中都参与了两侧对称花型的发育, 但它们的具体功能有明显的 差异。通过在豌豆花瓣中建立农杆菌EHA105介导的瞬时表达系统, 观察到豆科植物百脉根和玄参科植物金鱼草的CYC类 TCP 蛋白亚细胞定位有明显区别。

关键词：农杆菌; 瞬时表达; CYC类TCP蛋白; 亚细胞定位

收稿：2009-12-22   修定：2010-04-28

资助：国家高技术研究发展计划(“863” 计划) (2007AA10Z113)。

Using Agroinjection as a Transient Expression System to Investigate Subcellular Localization of CYC-like TCP Proteins

LI Chao¹, XU Zhi-Yong², YANG Jun², LUO Da^1,2,*

¹School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China; ²National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

Corresponding author: LUO Da; E-mail: dluo@sibs.ac.cn; Tel: 021-54924108

Abstract:

CYCLOIDEA-like TCP genes control the floral zygomorphy in both legume and Scrophulariaceae plants with divergent functions. A transient expression system using agroinjection mediated by Agrobacterium tumefaciens strain EHA105 was established in petals of pea (Pisum sativum L.), and we found that the subcellular localizations of two homologous proteins, CYCLOIDEA from Antirrhinum majus and LjCYC1 from Lotus japonicus, are differentially distributed in cells.

Key words: Agrobacterium tumefaciens; transient expression; CYC-like TCP proteins; subcellular localization